Authors: Y. De Ladurantaye, M. Khelifi, C. Cloutier And T. A. Coudron
Identifier: C0814
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Published in: CBE Journal » CBE Journal Volume 52 (2010)

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Description: The most common method of controlling the Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is the application of chemical insecticides. Several alternatives to chemical insecticides have been proposed, but are in general not yet applicable for current commercial scale production. One of them is augmentative biological control using a natural insect predator. Consideringthe need of more sustainable agriculture, the objective of this study was to develop adequate short-term storage conditions for the second instar nymphs of the predaceous pentatomid, Perillus bioculatus. This instar has previously been found to be suitable for release to control eggs and early instars of the CPB in the springon potatoes. Many laboratory tests on P. bioculatus second instar nymphs have been carried out to investigate the followingstorag e conditions: air temperature, photoperiod, and period of storage. Results show that storage temperatures within the range of 9 to 15oC and photoperiod (16:8 h L:D and 0:24 h L:D) did not significantly affect the survival and post-storage development of P. bioculatus nymphs stored for periods of 2 to 8 d. Compared with shorter periods (i.e., less than 8 days), storage for 10 days significantly reduced the survival rate by about 20%. Development time to the next instar at 208C followingstorag e was not significantly affected by experimental conditions, although development was slightly faster at 158C for 10 days of storage. Future studies should investigate the dispersal and predaceous performance of P. bioculatus followingvarious combinations of storage conditions.

Keywords: storage, temperature, photoperiod, colorado potato beetle, chemical insecticides, alternative, biological control, perillus bioculatus.
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Date: 2010-07-20
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Publication type: Journal
Coverage: Canada
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