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Identifier: CSBE16027
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Published in: CSBE-SCGAB Technical Conferences » AGM Halifax 2016

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Description: Studies at a single cellular level are superior to bulk population based methods, as the results are not subject to characteristics inherent in large bacterial environments. Properties of single bacterium, electrical conductivity in particular, play a significant role in specific and quantitative detection. Using atomic force microscopy and it’s modules to probe properties and provide a thorough understanding of single bacterial cells, would enhance development of novel biosensors. Additionally, the role of electrical currents and surface potential in the detachment and inactivation of bacterial cells adhered to surfaces can aid in the development of strategies for antifouling tools. Knowledge of an individual cell’s ability to respond to its environment, along with its interaction with surrounding cells or its host, is essential in order to develop new solutions to inhibit or promote the growth of bacteria or biofilms. The overall goal of this project is to investigate a single bacterial cell’s resistive properties, adhesion kinetics and motility mechanisms on various substrates using conductive scanning probe microscopy (with ResiScope module) and microfluidic platforms. Through a microbial ecology approach, at spatiotemporal and pathological scales, we provide results on the investigation of the interactions of polymicrobial communities using nanoscale imaging techniques and microfluidic platforms. The bridging of microfluidic techniques along with nanoscale imaging, allows for real time observation of individual cells within populations.

Keywords: ResiScope TM, Conductive scanning probe microscopy, Bacterial adhesion, Microfluidics, Antifouling
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Citation: . 2016. Investigation of the Individual Microbe Single Cell Analysis Using BioNano Tools. CSBE/SCGAB 2016 Annual Conference, Halifax, 3-6 July 2016.
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Pages -
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Date: juil-16
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Conference name: CSBE/SCGAB 2016 Annual Conference, Halifax, 3-6 July 2016.
Session name: Session 1C: Information Technologies and Bio-instrumentation

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Type: Text.Article
Format: PDF
Publication type: Conference Proceeding
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Coverage: Canada
Language 1: en
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Rights: Canadian Society for Bioengineering
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