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- Consistent monitoring and analysis of reactor operation and microbial community composition over five years delivers insight into the stability and resilience of an anaerobic dairy manure digester
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Consistent monitoring and analysis of reactor operation and microbial community composition over five years delivers insight into the stability and resilience of an anaerobic dairy manure digester
Authors: Jordaan, Elsie Maria, Cicek, Nazim, Derakhshani, Hooman, Khafipour, Ehsan
Identifier: CSBE17040
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Published in: CSBE-SCGAB Technical Conferences » AGM Winnipeg 2017 (with CIGR VI Technical Symposium)
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Description: The use of high-throughput sequencing methods to characterize microbial communities in anaerobic digestion systems is becoming more frequent, yet little is known about the long-term stability and evolution of such communities. Over the course of more than 1650 days the operational parameters of a bench-scale mesophilic dairy manure digester were continually monitored with periodic samples taken for microbial analysis. The batch-fed digester went through four distinct disturbances, preceded and followed by five periods of steady state operation, each lasting between 150 and 330 days. The disturbances were the result of using manure feed batches that had been modified due to normal dairy operation, either through changing the feeding regimen to include dried distillers grains and solubles (DDGS) or by a recent application of copper sulfate footbath solution. While the addition of DDGS increased biogas production, the presence of copper sulfate inhibited biogas production and led to a significant increase in VFAs. Steady state conditions were re-established by using regular manure as feedstock. Initial analysis of sequenced 16S rRNA genes revealed a diverse bacterial community that maintained a fairly stable steady state composition with distinct changes occurring mainly during periods of disturbance. Methanosarcina dominated the archaeal community during steady state periods, while compositional changes during disturbances were largely attributed to inhibition of methanogens. Upcoming analysis aims to determine whether the dairy manure digester maintained a core group of bacteria and archaea during all steady state periods despite the various disturbances disrupting community composition.
Keywords: Anaerobic digestion, Illumina sequencing, community stability, dairy manure
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Date: 2017-08-07
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Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Session 3A: Biodiesel, Biogas, and Pre-treatment
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Type: Text.Article
Format: PDF
Publication type: Technical conference
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Coverage: Canada
Language 1: en
Language 2:
Rights: Canadian Society for Bioengineering
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DOI:
Description: The use of high-throughput sequencing methods to characterize microbial communities in anaerobic digestion systems is becoming more frequent, yet little is known about the long-term stability and evolution of such communities. Over the course of more than 1650 days the operational parameters of a bench-scale mesophilic dairy manure digester were continually monitored with periodic samples taken for microbial analysis. The batch-fed digester went through four distinct disturbances, preceded and followed by five periods of steady state operation, each lasting between 150 and 330 days. The disturbances were the result of using manure feed batches that had been modified due to normal dairy operation, either through changing the feeding regimen to include dried distillers grains and solubles (DDGS) or by a recent application of copper sulfate footbath solution. While the addition of DDGS increased biogas production, the presence of copper sulfate inhibited biogas production and led to a significant increase in VFAs. Steady state conditions were re-established by using regular manure as feedstock. Initial analysis of sequenced 16S rRNA genes revealed a diverse bacterial community that maintained a fairly stable steady state composition with distinct changes occurring mainly during periods of disturbance. Methanosarcina dominated the archaeal community during steady state periods, while compositional changes during disturbances were largely attributed to inhibition of methanogens. Upcoming analysis aims to determine whether the dairy manure digester maintained a core group of bacteria and archaea during all steady state periods despite the various disturbances disrupting community composition.
Keywords: Anaerobic digestion, Illumina sequencing, community stability, dairy manure
Résumé:
Mots-clés:
Citation:
Volume:
Issue:
Pages -
Contributor:
Date: 2017-08-07
Technical field:
Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Session 3A: Biodiesel, Biogas, and Pre-treatment
Other information:
Type: Text.Article
Format: PDF
Publication type: Technical conference
Source:
Relation:
Coverage: Canada
Language 1: en
Language 2:
Rights: Canadian Society for Bioengineering
Notes:
Other files:
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