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Description: Increasing productivity of microbial processes targeting production of value-added bio-products is a major focus of bioprocess development. For medium chain length polyhydroxyalkanoates (mcl-PHAs), a potentially valuable biodegradable and biocompatible bio-polymer, this is often done using high cell density cultures. During such processes, gas-to-liquid mass transfer of oxygen is often rate limiting, and the mass transfer coefficient is increased through bioreactor aeration and mixing intensity. We have observed that during high cell density cultivation of Pseudomonas putida LS46 the rheological properties of the cultivation medium change significantly over time, with more than a ten-fold increase in apparent viscosity (at a shear rate of 10 sec-1) for cell densities ranging 0-25 g/L CDM. Shear thickening behavior was observed for all samples beyond a certain minimum yield shear rate of 95 sec-1. Up to that point the behavior of samples with a cell concentration of less than 10 g/L CDM was approximately Newtonian (<10 g/L CDM) and slightly shear thinning for cell densities beyond 15 g/L CDM. Many inverse correlations have been previously established between medium viscosity and kLa. This data indicates that increasing the bioreactor mixing intensity beyond the onset of shear thickening could have a detrimental effect on kLa, and productivity, during high cell density mcl-PHA cultivation processes.

Keywords: Renewable biomaterials, Bioplastics, Polyhydroxyalkanoates, Pseudomonas putida
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Date: 2017-08-07
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Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Poster Session

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Type: Text.Article
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Publication type: Technical conference
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Coverage: Canada
Language 1: en
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Rights: Canadian Society for Bioengineering
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