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Synthesis of Polyhydroxyalkanoates (PHAs) production from vegetable oil by Pseudomonas chlororaphis strains PA23 and PA23-63
Authors: Sharma, Parveen K., Munir, Riffat, Levin, David B.
Identifier: CSBE17017
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Published in: CSBE-SCGAB Technical Conferences » AGM Winnipeg 2017 (with CIGR VI Technical Symposium)
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Description: Polyhydroxyalkanoates (PHAs) have attracted extensive interest as environmentally friendly biodegradable plastics. Many bacteria synthesize short (SCL) and medium chain length (MCL) PHA polymers as carbon and energy storage reserves when they are grown under nutrient-limitation Characteristics of MCL-PHA resemble those of elastomers, latexes, and resins. Pseudomonas chlororaphis strain PA23 was isolated from soybean root and was able to protect canola from stem rot disease caused by the fungal pathogen Sclerotinia sclerotiorum. This bacterium secretes a wide-range of compounds, including the antibiotics pyrrolnitrin, phenazine 1-carboxylic acid (PCA), and 2-hydroxyphenazine (2-OH-PHZ), together with chitinase, protease, lipase, and siderophores. Synthesis of PCA conveys this strain with a bright orange color. P. chlororaphis PA23 can grow octanoic, nonanoic acid as well as in canola oil and produced 1.56-2.61 g/L cell dry weight and accumulated PHAs from 10.6% to 28.3% of cell dry weight (cdw). P. chlororaphis PA23-63 is a non-pigmented strain derived from PA23 that does not synthesize PCA. We have determined that P. chlororaphis PA23-63 was also able produce 2.42-5.14 g/L cell biomass and accumulated PHA from 11.7% to 32.5% of cdw from octanoic acid, nonanoic acid, waste fryer oil, and canola oil. Recombinants of P. chlororaphis PA23-63 are being constructed to produce PHAs with novel monomer composition from directly from vegetable oils.
Keywords: Renewable biomaterials, Bioplastics, Polyhydroxyalkanoates, Pseudomonas putida
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Date: 2017-08-07
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Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Poster Session
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Type: Text.Article
Format: PDF
Publication type: Technical conference
Source:
Relation:
Coverage: Canada
Language 1: en
Language 2:
Rights: Canadian Society for Bioengineering
Notes:
Other files:
DOI:
Description: Polyhydroxyalkanoates (PHAs) have attracted extensive interest as environmentally friendly biodegradable plastics. Many bacteria synthesize short (SCL) and medium chain length (MCL) PHA polymers as carbon and energy storage reserves when they are grown under nutrient-limitation Characteristics of MCL-PHA resemble those of elastomers, latexes, and resins. Pseudomonas chlororaphis strain PA23 was isolated from soybean root and was able to protect canola from stem rot disease caused by the fungal pathogen Sclerotinia sclerotiorum. This bacterium secretes a wide-range of compounds, including the antibiotics pyrrolnitrin, phenazine 1-carboxylic acid (PCA), and 2-hydroxyphenazine (2-OH-PHZ), together with chitinase, protease, lipase, and siderophores. Synthesis of PCA conveys this strain with a bright orange color. P. chlororaphis PA23 can grow octanoic, nonanoic acid as well as in canola oil and produced 1.56-2.61 g/L cell dry weight and accumulated PHAs from 10.6% to 28.3% of cell dry weight (cdw). P. chlororaphis PA23-63 is a non-pigmented strain derived from PA23 that does not synthesize PCA. We have determined that P. chlororaphis PA23-63 was also able produce 2.42-5.14 g/L cell biomass and accumulated PHA from 11.7% to 32.5% of cdw from octanoic acid, nonanoic acid, waste fryer oil, and canola oil. Recombinants of P. chlororaphis PA23-63 are being constructed to produce PHAs with novel monomer composition from directly from vegetable oils.
Keywords: Renewable biomaterials, Bioplastics, Polyhydroxyalkanoates, Pseudomonas putida
Résumé:
Mots-clés:
Citation:
Volume:
Issue:
Pages -
Contributor:
Date: 2017-08-07
Technical field:
Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Poster Session
Other information:
Type: Text.Article
Format: PDF
Publication type: Technical conference
Source:
Relation:
Coverage: Canada
Language 1: en
Language 2:
Rights: Canadian Society for Bioengineering
Notes:
Other files:
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