- You are here:
-
-
All publications
- Synthesis and physical properties of polyhydroxyalkanoate polymers with novel monomer compositions by recombinant Pseudomonas putida LS46 expressing a novel PHA Synthase (PhaC116) enzyme
All publications from your selection
Synthesis and physical properties of polyhydroxyalkanoate polymers with novel monomer compositions by recombinant Pseudomonas putida LS46 expressing a novel PHA Synthase (PhaC116) enzyme
Authors: Levin, David B., Sharma, Parveen K., Munir, Riffat, Blunt, Warren, Dartiailh, Chris, Cheng, Juijun, Charles, Trevor
Identifier: CSBE17015
Download file:
Published in: CSBE-SCGAB Technical Conferences » AGM Winnipeg 2017 (with CIGR VI Technical Symposium)
Download RAW file:
DOI:
Description: A derivative of Pseudomonas putida LS461 (deletion the phaC1-phaZ-phaC2 genes) was constructed by introducing cosmid JC123 carrying a novel phaC116 gene from a metagenomic clone. The resulting strain, P. putida LS46123, was able to synthesize polyhydroxyalkanoate (PHA) polymers with novel monomer compositions when cultured on glucose or free fatty acids, and accumulated PHAs from 9.24 to 27.09% of cell dry weight. The PHAs synthesized by P. putida LS46123 contained up to 50 mol% short chain length subunits (3-hydroxybutyrate and 3-hydroxyvalerate), with the remaining monomers consisting of various medium chain length subunits. The PhaC116 protein expressed by P. putida LS46123 had an amino acid sequence identity of 45% with the PhaC1 protein of the parent strain, P. putida LS46. Predicted 3-D structures of the PhaC116 proteins from P. putida LS46123 and P. putida LS46 revealed several differences in the numbers and locations of protein secondary structures. The physical and thermal properties of the novel polymers synthesized by P. putida LS46123 cultured with glucose or free fatty acids differed significantly from those produced by P. putida LS46 grown on the same substrates. PHA polymers with different subunit compositions, and hence different physical and thermal properties can be tailor made for specific applications.
Keywords: Renewable biomaterials, Bioplastics, Polyhydroxyalkanoates, Pseudomonas putida
Résumé:
Mots-clés:
Citation:
Volume:
Issue:
Pages -
Contributor:
Date: 2017-08-07
Technical field:
Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Poster Session
Other information:
Type: Text.Article
Format: PDF
Publication type: Technical conference
Source:
Relation:
Coverage: Canada
Language 1: en
Language 2:
Rights: Canadian Society for Bioengineering
Notes:
Other files:
DOI:
Description: A derivative of Pseudomonas putida LS461 (deletion the phaC1-phaZ-phaC2 genes) was constructed by introducing cosmid JC123 carrying a novel phaC116 gene from a metagenomic clone. The resulting strain, P. putida LS46123, was able to synthesize polyhydroxyalkanoate (PHA) polymers with novel monomer compositions when cultured on glucose or free fatty acids, and accumulated PHAs from 9.24 to 27.09% of cell dry weight. The PHAs synthesized by P. putida LS46123 contained up to 50 mol% short chain length subunits (3-hydroxybutyrate and 3-hydroxyvalerate), with the remaining monomers consisting of various medium chain length subunits. The PhaC116 protein expressed by P. putida LS46123 had an amino acid sequence identity of 45% with the PhaC1 protein of the parent strain, P. putida LS46. Predicted 3-D structures of the PhaC116 proteins from P. putida LS46123 and P. putida LS46 revealed several differences in the numbers and locations of protein secondary structures. The physical and thermal properties of the novel polymers synthesized by P. putida LS46123 cultured with glucose or free fatty acids differed significantly from those produced by P. putida LS46 grown on the same substrates. PHA polymers with different subunit compositions, and hence different physical and thermal properties can be tailor made for specific applications.
Keywords: Renewable biomaterials, Bioplastics, Polyhydroxyalkanoates, Pseudomonas putida
Résumé:
Mots-clés:
Citation:
Volume:
Issue:
Pages -
Contributor:
Date: 2017-08-07
Technical field:
Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Poster Session
Other information:
Type: Text.Article
Format: PDF
Publication type: Technical conference
Source:
Relation:
Coverage: Canada
Language 1: en
Language 2:
Rights: Canadian Society for Bioengineering
Notes:
Other files:
©
2025
CSBE-SCGAB