Authors: Karolin Dietrich,
Marie-Jos?e Dumont,
Val?rie Orsat,
Luis F. Del Rio
Identifier: CSBE19179
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Published in: CSBE-SCGAB Technical Conferences » AGM Vancouver 2019
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Description: While ?plastics? are commonly known for their accumulation and persistence in the environment, they are also important products in the transition to a carbon neutral economy. In petrorefineries, the high value and increasing market volume of plastics ensure economic success. Similarly, biorefineries increase their competitiveness by including biobased plastics in their product portfolios.
The compostable biopolymer polyhydroxybutyrate (PHB) is a type of bacterially produced polyester with similar material properties as polypropylene. To produce PHB in an integrated biorefinery, softwood chips were pretreated with a mild dilute acid treatment. This allows hemicellulose sugar extraction, while leaving cellulose and lignin for other product streams. Two extraction times (5 min and 15 min) yielded a similar total sugar (monosaccharides) concentration of 20 g/L, while differing in the inhibitor profile. The longer reaction time increased the concentration of inhibitory phenols, furans and volatile organic acids. To remove inhibitors, overliming, activated charcoal, membrane treatment and resin filtration were applied as detoxification methods. While the inhibitor concentrations decreased for both extraction times, only the hydrolysate of 5 min resulted in sugar consumptions and increase of cell dry mass during a 24h fermentations with the bacterial strain P. sacchari IPT 101.
Softwood hemicellulose is an attractive potential carbon source for PHA production if the inhibitor content can be managed. As a simple remedy, reducing the extraction time of wood hydrolysates prevents inhibitor formation and allows bacterial growth.
Keywords: Biorefinery, softwood, hemicellulose, inhibitors, PHB.
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Date: 2019-07-15
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Conference name: CSBE/SCGAB 2019 Annual Conference, Vancouver, BC, 14-17 July 2019.
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Type: Poster
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Publication type: Text.Abstract
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Coverage: Canada
Language 1: en
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Rights: Canadian Society for Bioengineering
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