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Lipid production in R. toruloides-1588 using C5 and C6 wood hydrolysate
Authors: Carlos Saul Osorio-Gonzalez, Krishnamoorthy Hegde, Rahul Saini, Satinder Kaur Brar, Alain Lefebvre, Antonio Avalos Ramirez
Identifier: CSBE21262
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Published in: CSBE-SCGAB Technical Conferences » 5th CIGR and AGM Quebec City 2021 » Regular Sessions
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Description: In this study, a response surface methodology with fifteen different treatments was used to evaluate the effect of different initial xylose: glucose, and C/N ratios, as well as a lipid inducer on sugar utilization and lipid production using Rhodosporidium toruloides-1588 and wood hydrolysates as a culture media. Total specific sugar consumption was 0.086 (g-1g-1h) and maximum lipid production of 5.3 g/L was observed in treatment condition at 1:1 initial glucose: xylose ratio, C/N=70, and 1.05 g/L of lipid inducer at 96 h of fermentation. Predominantly, monounsaturated fatty acid distribution was palmitic (33%), stearic (21%), oleic (7.5%), and linoleic (12%) acid. In addition, polyunsaturated fatty acids were found in low abundance (<2%) in all treatments. Furthermore, R. toruloides-1588 was capable of producing lipids in the presence of different inhibitor compounds, such as levulinic acid, 5-HMF, furfural, vanillin, vanillic acid, syringaldehyde, ferulic acid, and 4-hydroxybenzoic acid. The study has shown that R. toruloides-1588 is a suitable microorganism to produce lipids using wood hydrolysate as a substrate.
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Date: 2021-06-11
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Conference name: 5th CIGR International Conference and CSBE-SCGAB AGM 2021, Quebec City,QC, 11-14 May 2021.
Session name: Energy 1 - Thermochemical & Bio Processes
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Type: Text.Abstract
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Publication type: Presentation
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Coverage: Canada
Language 1: en
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Rights: Canadian Society for Bioengineering
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DOI:
Description: In this study, a response surface methodology with fifteen different treatments was used to evaluate the effect of different initial xylose: glucose, and C/N ratios, as well as a lipid inducer on sugar utilization and lipid production using Rhodosporidium toruloides-1588 and wood hydrolysates as a culture media. Total specific sugar consumption was 0.086 (g-1g-1h) and maximum lipid production of 5.3 g/L was observed in treatment condition at 1:1 initial glucose: xylose ratio, C/N=70, and 1.05 g/L of lipid inducer at 96 h of fermentation. Predominantly, monounsaturated fatty acid distribution was palmitic (33%), stearic (21%), oleic (7.5%), and linoleic (12%) acid. In addition, polyunsaturated fatty acids were found in low abundance (<2%) in all treatments. Furthermore, R. toruloides-1588 was capable of producing lipids in the presence of different inhibitor compounds, such as levulinic acid, 5-HMF, furfural, vanillin, vanillic acid, syringaldehyde, ferulic acid, and 4-hydroxybenzoic acid. The study has shown that R. toruloides-1588 is a suitable microorganism to produce lipids using wood hydrolysate as a substrate.
Keywords:
Résumé:
Mots-clés:
Citation:
Volume:
Issue:
Pages -
Contributor:
Date: 2021-06-11
Technical field:
Conference name: 5th CIGR International Conference and CSBE-SCGAB AGM 2021, Quebec City,QC, 11-14 May 2021.
Session name: Energy 1 - Thermochemical & Bio Processes
Other information:
Type: Text.Abstract
Format:
Publication type: Presentation
Source:
Relation:
Coverage: Canada
Language 1: en
Language 2:
Rights: Canadian Society for Bioengineering
Notes:
Other files:
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