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Until recently, researchers reporting corn kernel damage levels, from field or laboratory threshing studies, have ex pressed the damage levels as a percentage by weight. The amount of damaged corn was evaluated by sieving, visual sorting of the damaged kernels, or a combination of both. These methods can be time consuming and do not accurately account for the fact that kernel damage may occur on a continuous scale. A colorimetric technique has been developed by Chowdhury and Buchele (1976) and Chowdhury et al. (1976). The technique consists of staining a sample of corn kernels with Fast Green FCF dye the dye reacts only with the exposed internal tissue of the corn kernel. Excess dye is rinsed off with distilled water and the sample is then placed in a mild sodium hydroxide solution which dissolves the dye adhering to the damaged kernels. The percentage transmittance or absorbance of the solution is then measured using a colimeter. Clearly, the greater the damage levels in a sample, the greater the dye concentration in the NaOH solution and therefore the greater the absorbance. Chowdhury (1978) discussed the development of a scale to report kernel damage evaluated using the colorimetric technique. One may create a scale or' calibration curve
a technical note on the use of a colorimetric technique for evaluating mechanical corn kernel damage
Wall, G. L. and E. R. Norris 1981. A TECHNICAL NOTE ON THE USE OF A COLORIMETRIC TECHNIQUE FOR EVALUATING MECHANICAL CORN KERNEL DAMAGE. Canadian Agricultural Engineering 23(1):67-68.
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