Lipase-catalyzed synthesis of glycerides via poly-esterification of free n-3 fatty acids in a solvent system
Description: Dietary ?-3 fatty acids are implicated as critical nutrients in the development of eye and brain function in infants. Epidemiological and intervention studies have shown that by increasing the intake of dietary ?-3 fatty acids, the risk of coronary heart disease is decreased. The use of lipases on the enrichment and incorporation of n-3 fatty acids into glyceride structure as well as the lipase regioselectivity and affinity for different substrates have been explored. Due to its similar activity towards the fatty acids having a different chain length and unsaturation degree, Candida antarctica lipase fraction B, has been successfully used for the production of n-3 fatty acids enriched glycerides. In this work, the effects of temperature (35 to 60?C) and free fatty acids/glycerol ratio (1.5 to 2.5) on glyceride production were tested. The reaction was catalyzed by Candida antarctica lipase fraction B by a polyesterification reaction from glycerol and a preparation of n-3 enriched free fatty acids from fish oil in a solvent system. According to our results, the production of triacylglycerols, 1,3-diacylglycerols and 1(3)-monoacylglicerols was affected by substrates molar ratio, while the production of triacylglycerols and 1(3)-monoacylglycerols was affected by both, substrates molar ratio and reaction temperature. Under our experimental conditions, the main product obtained were triacylglycerols with a concentration up to 0.9178 mM at 64
Keywords: candida antarctica fraction b, n-3 fatty acids, glycerides, esterification reaction.
Technical field: technical_fields_app4
Session name: Food and bioprocess engineering