Optimization of growth parameters of kluyveromyces lactis k7 for human lysozyme production in biofilm reactor

Authors: Ercan, D, A Demirci
Description: Lysozyme (1,4-?-N-acetylmuramidase) is a lytic enzyme, which degrades a constituent of bacterial cell wall. Lysozyme has been of interest in medicine, cosmetics, and food industries because of its anti-bactericidal effect. Several approaches for the production of human lysozyme have been reported. Kluyveromyces lactis is a suitable host cell for the production of human lysozyme because it has effective secretory capacity and crabtree negative effect in fully oxidative conditions. K. lactis K7 is a genetically modified organism that expresses human lysozyme. There is a need to improve the human lysozyme production by K. lactis K7 to make the human lysozyme more affordable. The aim of this study was to determine the optimal plastic composite support (PCS) and growth conditions of K. lactis K7 for the human lysozyme production in biofilm reactor with PCS. The best PCS type was selected according to the biofilm formation on PCS (CFU/g) and human lysozyme production (Unit/ml). After determination of optimal plastic composite support in test tubes, a bioreactor equipped with a 2 L vessel was implemented. To find the best combination of growth parameters, three factors Box
Keywords: human lysozyme, kluyveromyces lactis k7, biofilm reactor, plastic composite support, growth parameters
Technical field: technical_fields_app4
Session name: Food and bioprocess engineering
Date: 2012
Identifier: CSBE12021

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