Testing of properties for hexane extracts of stems of canola, hemp, flax, and sweet clover
Authors: Thakur, S., J. Morrison, Y. Chen
Description: As microorganisms become resistant to synthesized antibiotics it is our choice to examine constituents of plant parts to discover new antimicrobials. Since medieval times all portions of plants were used medicinally. However, this project focused on field crop waste streams such as stems. Plant tissues, including stems, possess secondary metabolites, including essential oils, and polyphenols, which have known antibacterial and antioxidant properties. The main purpose of this study was to examine antimicrobial activity due to the presence of secondary metabolites in stem extracts of canola, hemp, flax, and sweet clover. Soxhlet extraction was used for preparing stem extracts using hexane as a solvent. Antimicrobial activity was tested using suspension tests and all the dilutions will be plated for counting colony-forming units. Water sorption will be determined using "Dynamic Vapor Sorption Advantage" equipment, which uses how much and how fast a vapor is absorbed by the sample over time. FTIR spectra of stem extracts will be used to identify antioxidants using ATR-FTIR Aldrich database. The results of the experiments will be presented at the conference.
Keywords: stem extracts, plant secondary metabolite, antimicrobial activity, suspension, DVS, FTIR-ATR
Technical field: Bioprocess Systems Engineering
Conference name: CSBE/SCGAB 2015 Annual Conference, Edmonton, AB, 5-8 July 2015.
Session name: Bioprocessing III - Bio-material Properties and Processing
Citation: Thakur, S., J. Morrison, Y. Chen. 2015. Testing of properties for hexane extracts of stems of canola, hemp, flax, and sweet clover. CSBE/SCGAB 2015 Annual Conference, Edmonton, AB, 5-8 July 2015.
Publisher: Canadian Society for Bioengineering
Language 1: en
Rights: Canadian Society for Bioengineering