Rapid Detection of Avian Flu Using a Graphene Oxide Nano-Biosensor
Description: Currently, there are no on-farm tools available for rapid detection of influenza virus from poultry farms. Most of the time the analyte samples collected from throat of live animals, fecal content and blood components from the poultry location is transported to the centralized laboratory for analysis. Lack of field deployable tool causes significant delays in the time to results, leading to hindrances in containing the pandemics. To address this significant gap, we have developed an electrochemical based dual sensor platform composed of methylene blue-electroadsorbed graphene oxide nanostructures modified with monoclonal antibodies, H5N1 and H1N1. Bio-functional layers comprised of chitosan and protein-A molecules were implemented at the interface of sensor element and antibodies, which synergistically enriched the bio-activity of immobilized antibodies for the immune complex formation. Immunosensor platform based on methylene blue elecroadsorbed on graphene oxide derivatives towards detection of influenza virus antigen is novel. Developed sensing mechanism on the electrode can simultaneously differentiate between a human and an avian based influenza strain (H5N1 vs H1N1). The proposed immunosensor design not only provide rapid analytical response time (<1 min) but also provide simplicity in fabrication and instrumentation which paves an attractive platform for on-farm monitoring of influenza viral infections.
Keywords: Dual immunosensor; H5N1 flu detection; H1N1 flu detection; Graphene oxide; Methylene blue; Electroadsorption
Conference name: CSBE/SCGAB 2016 Annual Conference, Halifax, 3-6 July 2016.
Session name: Poster Session (Regular)
Citation: . 2016. Rapid Detection of Avian Flu Using a Graphene Oxide Nano-Biosensor. CSBE/SCGAB 2016 Annual Conference, Halifax, 3-6 July 2016.
Publisher: Canadian Society for Bioengineering
Language 1: en
Rights: Canadian Society for Bioengineering