Development of an oxygen-limited high cell density cultivation process for Pseudomonas putida LS46 to increase biopolymer productivity

Authors: Blunt, Warren; Dartiailh, Chris; Sparling, Richard; Gapes, Daniel; Levin, David B.; Cicek, Nazim
Description: During production of medium chain length polyhydroxyalkanoates (mcl-PHA) from fatty acids using Pseudomonas putida LS46, it was recently shown that both the polymer content of the cell and synthesis rate are increased under microaerophilic conditions. However, that work was conducted in batch bioreactors with low overall volumetric productivity. In the present study, we have increased the volumetric productivity using an oxygen-limited fed batch strategy. A feeding method was developed in which the carbon source and nitrogen source were fed in small, accurate pulses in response to off-gas CO2 concentrations using a Titrimetric Off-Gas Analyzer (TOGA) sensor system. Carbon and nitrogen were fed to ensure balanced growth as long as the dissolved oxygen remained above 0% air saturation, and subsequently oxygen limited conditions were allowed to prevail in order to increase polymer content. Using this method with maximal mass transfer capability of the bioreactor (aeration at 2 VVM using compressed air, mixing at 1200 rpm), 27.3
Keywords: Renewable biomaterials, Bioplastics, Polyhydroxyalkanoates; Pseudomonas putida
Conference name: CSBE/SCGAB 2017 Annual Conference, Canad Inns Polo Park, Winnipeg, Manitoba, 6-10 August 2017.
Session name: Session 1A: Biopolymers: Production & Properties
Publisher: Canadian Society for Bioengineering
Date: 2017-08-07
Publication type:
  • Technical conference
Type: Text.Article
Format: PDF
Identifier: CSBE17016
Coverage: Canada
Language 1: en
Rights: Canadian Society for Bioengineering

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